| Long name: |
Cy3 Conjugated, PCNA (Tyr211) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
cy3 conjugation kit |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PCNA Tyr211 |
| Specificity: |
This is a highly specific antibody against PCNA Tyr211 |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr211 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
190-230/261 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human PCNA around the phosphorylation site of Tyr211 |
| Gene ID number: |
5111 |
| Swiss Prot: |
P12004 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
APEX2 activities, Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, Has to be loaded onto DNA in order to be able to stimulate APEX2, Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways, but not apurinic-apyrimidinic (AP) endonuclease, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion, Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
512, 550nm/570, 615nm |
| Properties: |
1 of the PCNA (Tyr211) Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PCNA (Tyr211) Conjugated |
| Short name: |
Conjugated, PCNA (Tyr211) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy3 |
| Alternative name: |
cyanine 3 coupled, PCNA (Tyr211) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8729 |
| Gene: |
PCNA |
More about : PCNA |
| Long gene name: |
proliferating cell nuclear antigen |
| Locus: |
20p12, 3 |
| Discovery year: |
1990-01-15 |
| GenBank acession: |
J04718 |
| Entrez gene record: |
5111 |
| Pubmed identfication: |
2565339 |
| Havana BLAST/BLAT: |
OTTHUMG00000031798 |