| Long name: |
ALEXA FLUOR 594 Conjugated, PCNA (Tyr211) Polyclonal Antibody |
| Also known as: |
Anti-PCNA Tyr211 PAb ALEXA FLUOR 594 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PCNA Tyr211 |
| Specificity: |
This is a highly specific antibody against PCNA Tyr211 |
| Modification: |
Phosphorylation |
| Modification Site: |
Tyr211 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
190-230/261 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human PCNA around the phosphorylation site of Tyr211 |
| Gene ID Number: |
5111 |
| Swiss Prot: |
P12004 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
APEX2 activities, Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, Has to be loaded onto DNA in order to be able to stimulate APEX2, Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways, but not apurinic-apyrimidinic (AP) endonuclease, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion, Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
590nm/617nm |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
594, ALEXA FLUOR®, Alexa Fluor |
| Conjugated: |
Alexa conjugate 1 |
| Gene target: |
PCNA (Tyr211) |
| Short name: |
PCNA (Tyr211) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 594 |
| Alternative name: |
ALEXA FLUOR 594, PCNA (Tyr211) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8729 |
| Gene: |
PCNA |
More about : PCNA |
| Long gene name: |
proliferating cell nuclear antigen |
| Locus: |
20p12, 3 |
| Discovery year: |
1990-01-15 |
| GenBank acession: |
J04718 |
| Entrez gene record: |
5111 |
| Pubmed identfication: |
2565339 |
| Havana BLAST/BLAT: |
OTTHUMG00000031798 |