| Long name: |
Biotin Conjugated, LAMP-1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
LAMP-1 |
| Specificity: |
This is a highly specific antibody against LAMP-1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
300-350/417 |
| Subcellular location: |
Lysosome, Lumenal |
| Source: |
KLH conjugated synthetic peptide derived from human LAMP-1 |
| Gene ID number: |
3916 |
| Swiss Prot: |
P11279 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI), Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, This mutant protein then cycles between the plasma membrane and the endosome, Upon cell activation, also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation, Lysosome associated membrane protein (LAMP1) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
CD17 antigen-like family member A, CD17a, LAMP-1, LAMP1, LGP12, Lysosome-associated membrane glycoprotein 1, Lysosome-associated membrane protein 1, LAMPA |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
LAMP-1 Conjugated |
| Short name: |
Conjugated, LAMP-1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, LAMP-1 (Antibody to) |
| Alternative technique: |
antibodies |