| Long name: |
Biotin Conjugated, MMP-8 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MMP-8 |
| Specificity: |
This is a highly specific antibody against MMP-8 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
262-312/467 |
| Subcellular location: |
Secreted, Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human MMP8 |
| Gene ID number: |
4317 |
| Swiss Prot: |
P22894 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
All collagenases cleave fibrillar collagens at one specific site resulting in generation of N terminal three quarter and C terminal one quarter fragments, II, III, MMP13, MMP8 degrades fibrillar collagens types I, MMP8 is heavily glycosylated, MMP8 is very similar to MMP1, Most cell types do not produce MMP8, The human MMP8 gene has the chromosomal location of 11q22, The substrate specificity of collagenases is variable: MMP1 degrades type III collagen more efficiently than type I or type II collagen, The zymogen is quickly activated to the 64 Kd form, Until recently, aggrecan, and the zymogen has a mass of 85 Kd, and this breaks down to a cascade of active forms, but it has also been detected in other cell types including arthritic chondrocytes and gingival fibroblasts, in turn degrades type II collagen 6 fold more efficiently than type I and type II collagens and displays almost 50 fold stronger gelatinolytic activity than MMP1 and MMP8, it was thought that MMP8 was produced exclusively by neutrophils, serpins and alpha 2 macroglobulin, sharing 57 % amino acid identity, whereas MMP8 is more potent in degrading type I collagen than type III or type II collagen, which then denature to gelatin at body temperature, 2-22, 3, Matrix Metalloproteinase 8 (MMP8) is also known as neutrophil collagenase and collagenase 2 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
CLG1, MMP-8, MMP8, Matrix metalloproteinase-8, Neutrophil collagenase, PMNL collagenase, PMNL-CL, HNC |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MMP-8 Conjugated |
| Short name: |
Conjugated, MMP-8 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, MMP-8 (Antibody to) |
| Alternative technique: |
antibodies |