| Long name: |
ALEXA FLUOR 488 Conjugated, MMP-8 Polyclonal Antibody |
| Also known as: |
Anti-MMP-8 PAb ALEXA FLUOR 488 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
488, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MMP-8 |
| Specificity: |
This is a highly specific antibody against MMP-8 |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
262-312/467 |
| Subcellular location: |
Secreted, Extracellular |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human MMP8 |
| Gene ID Number: |
4317 |
| Swiss Prot: |
P22894 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
All collagenases cleave fibrillar collagens at one specific site resulting in generation of N terminal three quarter and C terminal one quarter fragments, II, III, MMP13, MMP8 degrades fibrillar collagens types I, MMP8 is heavily glycosylated, MMP8 is very similar to MMP1, Most cell types do not produce MMP8, The human MMP8 gene has the chromosomal location of 11q22, The substrate specificity of collagenases is variable: MMP1 degrades type III collagen more efficiently than type I or type II collagen, The zymogen is quickly activated to the 64 Kd form, Until recently, aggrecan, and the zymogen has a mass of 85 Kd, and this breaks down to a cascade of active forms, but it has also been detected in other cell types including arthritic chondrocytes and gingival fibroblasts, in turn degrades type II collagen 6 fold more efficiently than type I and type II collagens and displays almost 50 fold stronger gelatinolytic activity than MMP1 and MMP8, it was thought that MMP8 was produced exclusively by neutrophils, serpins and alpha 2 macroglobulin, sharing 57 % amino acid identity, whereas MMP8 is more potent in degrading type I collagen than type III or type II collagen, which then denature to gelatin at body temperature, 2-22, 3, Matrix Metalloproteinase 8 (MMP8) is also known as neutrophil collagenase and collagenase 2 |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
499nm/519nm |
| Synonyms: |
CLG1, MMP-8, MMP8, Matrix metalloproteinase-8, Neutrophil collagenase, PMNL collagenase, PMNL-CL, HNC |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), Also Alexa Fluor 488 is pH stable, As a result of this photo stability, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is distinguished in the FL1 of a FACS-Calibur or FACScan, has a very high photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry, yet the MMP-8 Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
MMP-8 |
| Short name: |
MMP-8 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 488 |
| Alternative name: |
ALEXA FLUOR 488, MMP-8 (Antibody to) |
| Alternative technique: |
antibodies |