| Long name: |
FITC Conjugated, PAK3 (Ser139) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PAK3 Ser139 |
| Specificity: |
This is a highly specific antibody against PAK3 Ser139 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser139 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human PAK3 around the phosphorylation site of (Ser139) |
| Gene ID number: |
5063 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), CDC42 and RAC1, PAK 2, The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, The three most common isoforms are PAK 1, These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, and PAK 3 (also known as alpha PAK, and beta PAK, and caspase mediated proteolytic cleavage, and have been implicated in a wide range of biological activities, and may play a role in regulating the apoptotic events in the dying cell, catalyzed by Cdc42, gamma PAK, interaction with Src homology 3 (SH3) domains, is required for activation of PAK, members of which are activated by small molecular weight GTPases, respectively), P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
hPAK3 bPAK |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PAK3 (Ser139) Conjugated |
| Short name: |
FITC Conjugated, PAK3 (Ser139) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, PAK3 (Ser139) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8592 |
| Gene: |
PAK3 |
More about : PAK3 |
| Long gene name: |
p21 (RAC1) activated kinase 3 |
| Synonyms gene: |
MRX30 MRX47 |
| Synonyms gene name: |
X-linked 47 p21 (CDKN1A)-activated kinase 3 p21 protein (Cdc42/Rac)-activated kinase 3 , mental retardation |
| Locus: |
Xq23 |
| Discovery year: |
1998-03-25 |
| GenBank acession: |
AF068864 |
| Entrez gene record: |
5063 |
| Pubmed identfication: |
8826460 9731525 |
| RefSeq identity: |
NM_002578 |
| Classification: |
X-linked mental retardation |
| Havana BLAST/BLAT: |
OTTHUMG00000022202 |