| Antigen detectable with this ELISA kit: |
Interleukin 18 (IL18) |
| Species reactivity: |
Canine (Dog), Canis familiaris |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
9pg/mL, The minimum detectable dose of this kit is typically less than 5 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 18 (IL18), The concentration of Interleukin 18 (IL18) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 18 (IL18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Interleukin 18 (IL18), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Interleukin 18 (IL18) |
| Short name: |
Interleukin 18 (IL18) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Interleukin 18 (interleukin 18 (interferon-gamma-inducing factor)) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Extracellular, IGIF and IL-18 and IL-1g and IL1F4, IL18 and IDBG-630481 and ENSBTAG00000000277 and 281249, IL18 and IDBG-71390 and ENSG00000150782 and 3606, Il18 and IDBG-163502 and ENSMUSG00000039217 and 16173, cytokine activity, this GO :0000165 and MAPK cascade and biological process this GO :0001525 and angiogenesis and biological process this GO :0005125 and cytokine activity and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005829 and cytosol and cellular component this GO :0006954 and inflammatory response and biological process this GO :0006955 and immune response and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0030101 and natural killer cell activation and biological process this GO :0030155 and regulation of cell adhesion and biological process this GO :0030431 and sleep and biological process this GO :0031663 and lipopolysaccharide-mediated signaling pathway and biological process this GO :0032725 and positive regulation of granulocyte macrophage colony-stimulating factor production and biological process this GO :0032729 and positive regulation of interferon-gamma production and biological process this GO :0032740 and positive regulation of interleukin-17 production and biological process this GO :0032819 and positive regulation of natural killer cell proliferation and biological process this GO :0034105 and positive regulation of tissue remodeling and biological process this GO :0042033 and chemokine biosynthetic process and biological process this GO :0042088 and T-helper 1 type immune response and biological process this GO :0042092 and type 2 immune response and biological process this GO :0042094 and interleukin-2 biosynthetic process and biological process this GO :0042095 and interferon-gamma biosynthetic process and biological process this GO :0042104 and positive regulation of activated T cell proliferation and biological process this GO :0042231 and interleukin-13 biosynthetic process and biological process this GO :0042253 and granulocyte macrophage colony-stimulating factor biosynthetic process and biological process this GO :0042346 and positive regulation of NF-kappaB import into nucleus and biological process this GO :0045087 and innate immune response and biological process this GO :0050729 and positive regulation of inflammatory response and biological process this GO :0051142 and positive regulation of NK T cell proliferation and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071407 and cellular response to organic cyclic compound and biological process, this GO :0005125 : cytokine activity, this GO :0005125 : cytokine activity, interleukin 18 (interferon-gamma-inducing factor) |
| Identity: |
5986 |
| Gene: |
IL18 |
More about : IL18 |
| Long gene name: |
interleukin 18 |
| Synonyms gene name: |
interleukin 18 (interferon-gamma-inducing factor) |
| Synonyms: |
IGIF IL1F4 IL-1g IL-18 |
| Synonyms name: |
interferon-gamma-inducing factor |
| Locus: |
11q23, 1 |
| Discovery year: |
1997-07-25 |
| GenBank acession: |
U90434 |
| Entrez gene record: |
3606 |
| Pubmed identfication: |
7477296 9693051 |
| RefSeq identity: |
NM_001562 |
| Classification: |
Interleukins |
| Havana BLAST/BLAT: |
OTTHUMG00000167006 |