| Antigen detectable with this ELISA kit: |
Insulin (INS) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
123, 123, 370, 1111, 3333, 11pg/mL, 33pg/mL, 37pg/mL, 46-10000pg/mL The standard curve concentrations used for the ELISA&rsquo, 46pg/mL, s were 10000pg/mL |
| Sensitivity: |
9pg/mL, The minimum detectable dose of this kit is typically less than 47 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
2, 5 hoursours |
| Test principle: |
A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Insulin (INS) and unlabeled Insulin (INS) (Standards or samples) with the pre-coated antibody specific for Insulin (INS), After addition of the substrate solution, After incubation the unbound conjugate is washed off, An antibody specific for Insulin (INS) has been pre-coated onto a microplate, The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin (INS) in the sample, the intensity of color developed is reverse proportional to the concentration of Insulin (INS) in the sample, This assay employs the competitive inhibition enzyme immunoassay technique |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Insulin (INS) |
| Short name: |
Insulin (INS) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Insulin (insulin) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006521 and regulation of cellular amino acid metabolic process and biological process this GO :0006953 and acute-phase response and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0015758 and glucose transport and biological process this GO :0022898 and regulation of transmembrane transporter activity and biological process this GO :0030307 and positive regulation of cell growth and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0031018 and endocrine pancreas development and biological process this GO :0031904 and endosome lumen and cellular component this GO :0031954 and positive regulation of protein autophosphorylation and biological process this GO :0032148 and activation of protein kinase B activity and biological process this GO :0032270 and positive regulation of cellular protein metabolic process and biological process this GO :0032460 and negative regulation of protein oli this GO merization and biological process this GO :0032880 and regulation of protein localization and biological process this GO :0033861 and negative regulation of NAD(P)H oxidase activity and biological process this GO :0034774 and secretory granule lumen and cellular component this GO :0042060 and wound healing and biological process this GO :0042177 and negative regulation of protein catabolic process and biological process this GO :0042593 and glucose homeostasis and biological process this GO :0042802 and identical protein binding and molecular function this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0044267 and cellular protein metabolic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045429 and positive regulation of nitric oxide biosynthetic process and biological process this GO :0045597 and positive regulation of cell differentiation and biological process this GO :0045721 and negative regulation of gluconeogenesis and biological process this GO :0045725 and positive regulation of glycogen biosynthetic process and biological process this GO :0045740 and positive regulation of DNA replication and biological process this GO :0045818 and negative regulation of glycogen catabolic process and biological process this GO :0045821 and positive regulation of glycolytic process and biological process this GO :0045840 and positive regulation of mitosis and biological process this GO :0045861 and negative regulation of proteolysis and biological process this GO :0045908 and negative regulation of vasodilation and biological process this GO :0045909 and positive regulation of vasodilation and biological process this GO :0045922 and negative regulation of fatty acid metabolic process and biological process this GO :0046326 and positive regulation of glucose import and biological process this GO :0046628 and positive regulation of insulin receptor signaling pathway and biological process this GO :0046631 and alpha-beta T cell activation and biological process this GO :0046889 and positive regulation of lipid biosynthetic process and biological process this GO :0050708 and regulation of protein secretion and biological process this GO :0050709 and negative regulation of protein secretion and biological process this GO :0050715 and positive regulation of cytokine secretion and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050796 and regulation of insulin secretion and biological process this GO :0050995 and negative regulation of lipid catabolic process and biological process this GO :0051000 and positive regulation of nitric-oxide synthase activity and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051897 and positive regulation of protein kinase B signaling and biological process this GO :0055089 and fatty acid homeostasis and biological process this GO :0060266 and negative regulation of respiratory burst involved in inflammatory response and biological process this GO :0060267 and positive regulation of respiratory burst and biological process this GO :0090277 and positive regulation of peptide hormone secretion and biological process this GO :0090336 and positive regulation of brown fat cell differentiation and biological process this GO :1902176 and negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway and biological process this GO :2000252 and negative regulation of feeding behavior and biological process, Extracellular, IDDM1 and IDDM2 and ILPR and IRDN and MODY10, INS and IDBG-547491 and ENSG00000254647 and 3630, INS and IDBG-645238 and ENSBTAG00000013003 and 280829, identical protein binding, this GO :0000165 and MAPK cascade and biological process this GO :0002020 and protease binding and molecular function this GO :0002674 and negative regulation of acute inflammatory response and biological process this GO :0005158 and insulin receptor binding and molecular function this GO :0005159 and insulin-like growth factor receptor binding and molecular function this GO :0005179 and hormone activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0005796 and this GO lgi lumen and cellular component this GO :0006006 and glucose metabolic process and biological process this GO :0006112 and energy reserve metabolic process and biological process this GO :0006355 and regulation of transcription, this GO :0002020 : protease binding, this GO :0002020 : protease binding and also this GO :0005158 : insulin receptor binding and also this GO :0005159 : insulin-like growth factor receptor binding and also this GO :0005179 : hormone activity and also this GO :0005515 : protein binding and also this GO :0042802 : identical protein binding, this GO :0005158 : insulin receptor binding, this GO :0005159 : insulin-like growth factor receptor binding, this GO :0005179 : hormone activity, this GO :0005515 : protein binding, this GO :0042802 : identical protein binding, insulin |
| Identity: |
6081 |
| Gene: |
INS |
More about : INS |
| Long gene name: |
insulin |
| Synonyms gene: |
IDDM2 IDDM1 |
| Synonyms gene name: |
insulin-dependent diabetes mellitus 2 |
| Locus: |
11p15, 5 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
X70508 |
| Entrez gene record: |
3630 |
| Pubmed identfication: |
6243748 7773291 |
| RefSeq identity: |
NM_000207 |
| Classification: |
Endogenous ligands |
| Havana BLAST/BLAT: |
OTTHUMG00000009558 |