| Antigen detectable with this ELISA kit: |
Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
068ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase Inhibitor 1A (CDKN1A), The concentration of Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase Inhibitor 1A (CDKN1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Cyclin Dependent Kinase Inhibitor 1A (CDKN1A), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Description: |
Not all receptor antagonist that bind to enzymes are inhibitors, Since blocking an enzyme's activity can kill a pathogen , activator, caspase, esterase inhibitors , lipoprotein, many drugs are enzyme inhibitors, papain, pathway, peptidase, protease , proteinase, trypsin, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme, , Tissue, acrosin, activity, and decreases its , are , bind to enzymes and increase their , enzymatic activity, enzyme activator ligands or agonists , enzyme receptor , imbalance, metabolic , or correct a , or receptor ligands or receptor antagonists that bind to an , proteins |
| Gene target: |
Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) |
| Short name: |
Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Cip1)) Enzyme-linked immunosorbent assay reagent, Cyclin Dependent phosphorylation catalyst suppressor 1A (cyclin-dependent kinase inhibitor 1A (p21 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CAP20 and CDKN1 and CIP1 and MDA-6 and P21 and p21CIP1 and SDI1 and WAF1, CDKN1A and IDBG-630156 and ENSBTAG00000008353 and 513497, CDKN1A and IDBG-85033 and ENSG00000124762 and 1026, Cdkn1a and IDBG-159848 and ENSMUSG00000023067 and 12575, Cip1), metal ion binding, nuclei, signal transduction by p53 class mediator resulting in cell cycle arrest and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007265 and Ras protein signal transduction and biological process this GO :0007346 and regulation of mitotic cell cycle and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0009411 and response to UV and biological process this GO :0009636 and response to toxic substance and biological process this GO :0010033 and response to organic substance and biological process this GO :0010165 and response to X-ray and biological process this GO :0010243 and response to organonitrogen compound and biological process this GO :0010629 and negative regulation of gene expression and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0019912 and cyclin-dependent protein kinase activating kinase activity and molecular function this GO :0030308 and negative regulation of cell growth and biological process this GO :0030332 and cyclin binding and molecular function this GO :0030890 and positive regulation of B cell proliferation and biological process this GO :0031100 and organ regeneration and biological process this GO :0031668 and cellular response to extracellular stimulus and biological process this GO :0032403 and protein complex binding and molecular function this GO :0033158 and regulation of protein import into nucleus, this GO :0000079 and regulation of cyclin-dependent protein serine/threonine kinase activity and biological process this GO :0000082 and G1/S transition of mitotic cell cycle and biological process this GO :0000086 and G2/M transition of mitotic cell cycle and biological process this GO :0000278 and mitotic cell cycle and biological process this GO :0000307 and cyclin-dependent protein kinase holoenzyme complex and cellular component this GO :0004861 and cyclin-dependent protein serine/threonine kinase inhibitor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0006977 and DNA damage response, this GO :0004861 : cyclin-dependent protein serine/threonine kinase inhibitor activity, this GO :0004861 : cyclin-dependent protein serine/threonine kinase inhibitor activity and also this GO :0005515 : protein binding and also this GO :0019912 : cyclin-dependent protein kinase activating kinase activity and also this GO :0030332 : cyclin binding and also this GO :0032403 : protein complex binding and also this GO :0046872 : metal ion binding, this GO :0005515 : protein binding, this GO :0019912 : cyclin-dependent protein kinase activating kinase activity, this GO :0030332 : cyclin binding, this GO :0032403 : protein complex binding, this GO :0046872 : metal ion binding, translocation and biological process this GO :0034605 and cellular response to heat and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042326 and negative regulation of phosphorylation and biological process this GO :0042493 and response to drug and biological process this GO :0042771 and intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043068 and positive regulation of programmed cell death and biological process this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0045087 and innate immune response and biological process this GO :0045736 and negative regulation of cyclin-dependent protein serine/threonine kinase activity and biological process this GO :0045860 and positive regulation of protein kinase activity and biological process this GO :0046685 and response to arsenic-containing substance and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048146 and positive regulation of fibroblast proliferation and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0051384 and response to glucocorticoid and biological process this GO :0051412 and response to corticosterone and biological process this GO :0051726 and regulation of cell cycle and biological process this GO :0055093 and response to hyperoxia and biological process this GO :0060574 and intestinal epithelial cell maturation and biological process this GO :0070557 and PCNA-p21 complex and cellular component this GO :0071479 and cellular response to ionizing radiation and biological process this GO :0071850 and mitotic cell cycle arrest and biological process this GO :0071901 and negative regulation of protein serine/threonine kinase activity and biological process this GO :0090398 and cellular senescence and biological process this GO :0090400 and stress-induced premature senescence and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process this GO :2000278 and regulation of DNA biosynthetic process and biological process this GO :2000379 and positive regulation of reactive oxygen species metabolic process and biological process, cyclin-dependent kinase inhibitor 1A (p21 |
| Identity: |
1784 |
| Gene: |
CDKN1A |
More about : CDKN1A |
| Long gene name: |
cyclin dependent kinase inhibitor 1A |
| Synonyms gene: |
CDKN1 |
| Synonyms gene name: |
Cip1) , cyclin-dependent kinase inhibitor 1A (p21 |
| Synonyms: |
P21 CIP1 WAF1 SDI1 CAP20 p21CIP1 p21Cip1/Waf1 |
| Locus: |
6p21, 2 |
| Discovery year: |
1994-05-24 |
| GenBank acession: |
U03106 |
| Entrez gene record: |
1026 |
| RefSeq identity: |
NM_078467 |
| Havana BLAST/BLAT: |
OTTHUMG00000014603 |